Abstracts Biological Sciences

Respiration rates and Factors which Influence the levels of Carbohydrates and Lipids in Honey Bee Eggs ( Apis mellifera Linnaeus).

by Lucas Aloyse Mugah Mackasmiel

Respiration rates and changes in the amount of nutrients in queen-laid and worker-laid eggs of the honey bee, Apis mellifera L., were determined for the 3 days of embryonic development. Respiration was quantified by measuring the amount of CO<sub>2</sub> produced during 13 hr of artificial incubation at four temperature treatments: 28<sup>o</sup>C, 31<sup>o</sup>C, 34<sup>o</sup>C, 36<sup>o</sup>C (± 0.5<sup>o</sup>C). The amounts of lipids and carbohydrates were also quantified in the eggs of queens and laying workers on day 1, 2 and 3 using high performance thin layer chromatography. The mean respiration rate for fertilized and unfertilized eggs from queens was 0.1 ± 0.0 µL CO<sub>2</sub>/hr/egg, the same as the mean respiration rate obtained for unfertilized eggs from laying workers. The results of carbohydrate analysis showed a total of 2.4 ± 0.6 µg/egg total sugars in the fertilized eggs of queens, an equivalent of 8.3% on a dry weight basis, while unfertilized eggs contained a total of 1.4 ± 0.4 µg/egg total sugars, equivalent of 6.3% on a dry weight basis. Total lipids, excluding fatty acids, were 10.7 ± 6.1 µg/egg (37.4%) for fertilized eggs and 8.4 ± 1.3 µg/egg (40.8%) for unfertilized eggs. The respiration rate at 34<sup>o</sup>C was 0.17 ± 0.01 µL CO<sub>2</sub>/hr/egg on day 1, the same as day 1 at 36<sup>o</sup>C. Day 2 respiration rates were 0.13 ± 0.04 µL CO<sub>2</sub>/hr/egg and 0.15 ± 0.01 µL CO<sub>2</sub>/hr/egg, respectively. On day three, 0.22 ± 0.01 µL CO<sub>2</sub>/hr/egg was recorded at 34<sup>o</sup>C and 0.24 ± 0.01 µL CO<sub>2</sub>/hr/egg at 36<sup>o</sup>C. At low temperatures of 28<sup>o</sup>C and 31<sup>o</sup>C, a respiration rate of 0.12 ± 0.01 µL CO<sub>2</sub>/hr/egg was recorded on day 1, for eggs held at both temperatures. Day 2 results were 0.07± 0.01 µL CO<sub>2</sub>/hr/egg at 28<sup>0</sup>C and 0.11 ± 0.01 µL CO<sub>2</sub>/hr/egg at 31<sup>o</sup>C, while 0.07 ± 0.01 and 0.12 ± 0.01 µL CO<sub>2</sub>/hr/egg, respectively, were measured on day 3. Mortality results, as indicated by pre-emergence embryos, showed that 75% developed at 34<sup>o</sup>C compared to 37.5% at 36<sup>o</sup>C. Low temperatures of 28<sup>o</sup>C had 12.5% developing to pre-emergence stage while 50% developed at 31<sup>o</sup>C. Respiratory results showed significant differences (p=0.05) between the different days of incubation and temperature treatments, respectively. No significant difference was observed between the fertilized eggs and unfertilized eggs from queens at the same temperature treatment. The comparison of unfertilized eggs from queens and those from laying workers also showed no significant difference. The regression (R2=0.65) was significant (P=0.05) when CO<sub>2</sub> output on all the days and temperature treatments were compared. The amount of nutrients in the eggs of queens and those of laying workers, were significantly different (P=0.05) depending on egg type and age. No significant difference was observed between the colonies…